TransFect Protocol Database. and two new DNA transfection reagents: Attractene Transfection Reagent and NanoFect Transfection Reagent. I tried Lipofectamine and Attractene which both had a bad efficiency. plate format to do your experiment, the transfection protocol will be DNA/well. 年7月29日 QIAGEN Supplementary Protocol Protocol: Fast?Forward Transfection of cells with DNA using Attractene Transfection Reagent This.
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Cell strategies for high transfection efficiency
Incubate the samples for 10? Shortly before transfection, seed 3x cells per well of a 6-well plate in ? For this reason, further optimization is recommended to achieve maximum transfection efficiency.
Forward Transfection of cells with DNA using Attractene Transfection Reagent This protocol uses standard parameters and might require further optimization for transfection with this cell type. Make money from your old lab equipment.
If you require a thermal cycler that delivers high performance and high throughput, while still ret…. The table below shows a pipetting scheme with 5 different conditions we recommend to test when optimizing transfection i. Add the complexes drop? As a starting point, we recommend using 6-well plate? In this protocol, cell plating and transfection are performed on the same day.
Hygiena expands with PCR-based systems to cover the entire contamination detection spectrum Added: Cell strategies for high transfection efficiency 19 Feb by Qiagen. For these reasons, it is ideal for use when absence of animal-derived components is a priority, for example, in biopharmaceutical applications.
Home PCR Cell strategies for high tr However, for some cell types, especially difficult-to-transfect cells such as suspension cells and atttractene cells, higher transfection efficiencies may be achieved by plating cells 24 hours prior to transfection for a protocol, consult the Attractene Transfection Reagent Handbook at www. The TransFect Protocol Database is an invaluable resource which provides cell-specific transfection protocols.
Attraactene the medium as required.
Simple Safe Parallel Reaction Sampling. In addition, the absence of lipids makes this reagent suitable for lipid or signal transduction research. This reagent is suitable for a range of proyocol applications, including transient or stable transfection, transfection of shRNA short-hairpin RNA vectors, and DNA cotransfection. NanoFect Reagent is completely chemically synthesised, free of animal-derived components, and has been tested for the absence protoco endotoxins.
Ease of handling means that Attractene Reagent is suitable for use with automated systems. For stable transfections, passage cells into the appropriate selection medium 24? Use of the TransFect Database is free of charge and no registration is required. The amounts given are for one well of a 6-well plate. Absence of animal-derived components also facilitates regulatory compliance. Prime thermal cyclers provide flexible PCR Added: As a general rule, optimization of transfection can be performed by varying DNA and Attractene Reagent amount as follows: Using Attractene Reagent also ensures exceptionally low cytotoxicity.
The optimal incubation time for gene expression analysis depends on the cell type, the gene expressed, and the method of analysis. The effect of using greater or lesser amounts of DNA and Attractene Transfection Reagent can be observed using these combinations. The instrument range of Hygiena International Ltd, experts in rapid microbial detection, monitoring…. Incubate the cells with the transfection complexes under their normal growth conditions and monitor rpotocol expression after an appropriate time e.
Protoclo the reagent and nucleic acid amounts listed in the protocol. Routing Atrractene vs Ro Maintain cells in selective culture medium until colonies appear.